Saccharomyces cerevisiae is cultured in a chemostat (continuous fermentation) at a dilution rate of 0.5 h 1. The feed substrate concentration is 10 g.L 1. The biomass concentration in the chemostat at steady state will be _________g.L 1. Assumptions : Feed is sterile, maintenance is negligible and maximum biomass yield with respect to substrate is 0.4 (g biomass per g ethanol). Microbial growth kinetics is given by = m S Ks + Swhere is specific growth rate (h 1), m = 0.7 h 1, Ks = 0.3 g.L 1 and s is substrate concentration (g.L 1).

Saccharomyces cerevisiae is cultured in a chemostat (continuous fermen

1.	Saccharomyces cerevisiae is cultured in a chemostat (continuous fermentation) at a dilution rate of 0.5 h 1. The feed substrate concentration is 10 g.L 1. The biomass concentration in the chemostat at steady state will be _________g.L 1. Assumptions : Feed is sterile, maintenance is negligible and maximum biomass yield with respect to substrate is 0.4 (g biomass per g ethanol). Microbial growth kinetics is given by = m S Ks + Swhere is specific growth rate (h 1), m = 0.7 h 1, Ks = 0.3 g.L 1 and s is substrate concentration (g.L 1).
A.	1.15 to 2.25
B.	9.65 to 10.75
C.	3.65 to 3.75
D.	All of the above
Answer» D. All of the above

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