Pick out the wrong statement.

• A. Catalytic activity of enzyme catalysed reactions which is affected by temperature, pH value & chemical agents, is maximum at a temperature of about 45°C
• B. Most of the enzyme catalysed reactions involve at least two substrates
• C. Enzymes help in increasing the activation energy of the reaction
• D. Equilibrium concentrations in enzyme catalysed reactions can be calculated by using the thermodynamic properties of substrates & products